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Sustainable Chemical Processes

Table 3 SSF with steam exploded thatch grass involving various hydrolytic enzymes and Zymomonas mobilis

From: Scale up and efficient bioethanol production involving recombinant cellulase (Glycoside hydrolase family 5) from Clostridium thermocellum

SSF combination

Substrate concentration (w v -1) and Mode of SSF

Reducing sugar (g L-1)*

Ethanol yield (g of ethanol g of untreated substrate-1)

Ethanol titre (g L-1)*

Bacillus subtilis cellulase

1% Shake flask

1.10 ± 0.02

0.072

0.72 ± 0.02

Trichoderma reesei cellulase

1% Shake flask

1.10 ± 0.07

0.094

0.94 ± 0.05

Recombinant cellulase (GH5)

1% Shake flask

1.45 ± 0.03

0.120

1.20 ± 0.03

Recombinant cellulase (GH5)

5% Shake flask

12.10 ± 0.04

0.180

8.79 ± 0.04

Recombinant cellulase (GH5)

5% Bioreactor

18.98 ± 0.04

0.320

16.13 ± 0.03

  1. *the values correspond to the maximum reducing sugar and maximum ethanol titre at a particular time.
  2. Values are mean ± SE (n = 3).
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