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Sustainable Chemical Processes

Table 2 SHF with steam exploded thatch grass involving various hydrolytic enzymes and Zymomonas mobilis

From: Scale up and efficient bioethanol production involving recombinant cellulase (Glycoside hydrolase family 5) from Clostridium thermocellum

SSF combination

Substrate concentration (w v -1) and Mode of SSF

Reducing sugar (g L-1)*

Ethanol yield (g of ethanol g of untreated substrate-1)

Ethanol titre (g L-1)*

Bacillus subtilis cellulase

1% Shake flask

0.84 ± 0.05

0.051

0.51 ± 0.03

Trichoderma reesei cellulase

1% Shake flask

0.98 ± 0.06

0.067

0.67 ± 0.07

Recombinant cellulase (GH5)

1% Shake flask

1.21 ± 0.04

0.099

0.99 ± 0.02

  1. *the values correspond to the maximum reducing sugar and maximum ethanol titre at a particular time.
  2. Values are mean ± SE (n = 3).
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