Skip to main content

Table 1 Examples of fold improvements in promiscuous functions

From: Recent advances in enzyme promiscuity

Enzymes

Native function/substrate

Promiscuous functions/substrate

Improvement by directed laboratory evolution and/or rational designing

References

Mammalian serum paraoxonase

Lipo-lactonase

Phosphotriesterase CMP-Coumarine (racemic mixture)

77-fold higher

[35]

CMP-Coumarine (sp isomer)

86,000-fold

[39]

Phosphotriesterase (PTE) from bacteria

Paraoxon hydrolysis

Racemic nerve agent VX

230-fold

[42]

DEVX

78-fold

  

Malathion

5000-fold

[43]

PLL scaffold (Dr0930) from Deinococcus radiodurans

Lactonase

OP hydrolase

69,000-fold

[52]

Sortase A from Staphylococcus aureus

Transpeptidation of LPXTG

LAXTG and LPXSG

51,000-fold

[44]

Human glutathione transferase (GST) M2-2

2-cyano-1,3-dimethyl-1-nitrosoguanidine

Azathioprine

30-fold

[53]