Fig. 3From: Recent advances in enzyme promiscuityImprovement in promiscuous function of mammalian serum PON1. Several rounds of mutagenesis and selection by applying directed laboratory evolution and rational protein designing approaches resulted in the PON1 mutant exhibiting ~86,000-fold improvement in Kcat/Km values for the hydrolysis of stereospecific G-type nerve agent analogue Sp-CMP Coumarin. The selected clone had following mutations as compare to the starting gene -Ll69G, S111T, H115W, H134R, F222S and T332S, which are displayed in the figureBack to article page