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Fig. 3 | Sustainable Chemical Processes

Fig. 3

From: Recent advances in enzyme promiscuity

Fig. 3

Improvement in promiscuous function of mammalian serum PON1. Several rounds of mutagenesis and selection by applying directed laboratory evolution and rational protein designing approaches resulted in the PON1 mutant exhibiting ~86,000-fold improvement in Kcat/Km values for the hydrolysis of stereospecific G-type nerve agent analogue Sp-CMP Coumarin. The selected clone had following mutations as compare to the starting gene -Ll69G, S111T, H115W, H134R, F222S and T332S, which are displayed in the figure

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