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Table 1 Three phase partitioning of Thermomyces lanuginosus lipase (TLL) at 25 °C

From: Enhancement of stability of a lipase by subjecting to three phase partitioning (TPP): structures of native and TPP-treated lipase from Thermomyces lanuginosa

 

Protein (mg/mL)

Activity (U/mL)

Specific activity (U/mg protein)

Fold Increase

Assay in the absence of Triton X-100

 TLL

17.5

102

5.8

–

 TPP-TLL

0.22

0.72

3.3

0.57

Assay in the presence of Triton X-100

 TLL

17.5

151

8.6

–

 TPP-TLL

0.22

3.91

17.8

2.1

  1. The Thermomyces lanuginosus lipase was subjected to three phase partitioning as described in the materials and methods section. Experiments were carried out in triplicate and percentage error in each reading in a set was within 3 %. The activity was determined using pNPP assay and protein using bradford method