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Fig. 2 | Sustainable Chemical Processes

Fig. 2

From: Enhancement of stability of a lipase by subjecting to three phase partitioning (TPP): structures of native and TPP-treated lipase from Thermomyces lanuginosa

Fig. 2

Determination of the melting temperature of TPP-TLL using circular dichroism. Thermal denaturation curves were determined directly by monitoring the ellipticity changes at 222 nm. The samples with a concentration of 0.2 mg mL−1 were used. The temperature of sample solution was raised linearly by 1 °C min−1 from 50 to 100 °C. The heating curves were corrected for an instrumental baseline obtained by heating the buffer (10 mM sodium phosphate, pH 7.0) alone. The untreated lipase is shown with solid line and the TLL subjected to TPP is shown with dashed line

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