From: Biomolecular assembly strategies to develop potential artificial cellulosomes
Year | Artificial cellulosome | Scaffold-base | Enzyme type | Conjugation technique | Reference |
---|---|---|---|---|---|
2001 | First designer cellulosome | Engineered chimeric scaffoldin | Clostridium cellulolyticum, CelA, CelF | Cohesin-dockerin interaction | [3] |
2003 | Protein A- CBM -enzyme conjugates | Staphylococcal protein A | EGFP, Fusarium solani pisi lipase cutinase | IgG-affibody interaction | [4] |
2009 | Rosettazyme | Group II Chaperonin (Rosettazomes) | Clostridium thermocellum, Cel9B, Cel9K, Cel9R, Cel48S | Cohesin-dockerin interaction | [11] |
2011 | Nanocluster | Streptavidin-immobilized inorganic nanoparticles | Aspergillus niger, EglA | Avidin-biotin interaction | [12] |
2012 | Nanocluster | Polystyrene nanosphere | Trichoderma viride cellulase | EDC and Sulfo-NHS coupling chemistry | [13] |
2013 | Nanocluster | CdSe-ZnS core-shell quantum dots (QDs) | Clostridium thermocellum CelA, Clostridium cellulolyticum CelE | Metal affinity between core-shell QDs and polyhistidine tag | [14] |
2013 | Cellulase-ankyrin fusions | Ankyrin | Clostridium thermocellum CelA, Thermotoga marÃtima,, Cel12A | Protein fusion | [15] |
2013 | DNA-cellulase covalent conjugates | Double strand DNA | Thermobifida fusca, Cel5A | MTG mediated cross-linking | [16] |
2014 | DNA-cellulase non-covalent conjugates | Double strand DNA | Clostridium thermocellum, CelA | Zinc-finger protein guided assembly | [17] |