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Table 1 Design of artificial cellulosome for lignocellulose hydrolysis

From: Biomolecular assembly strategies to develop potential artificial cellulosomes

Year

Artificial cellulosome

Scaffold-base

Enzyme type

Conjugation technique

Reference

2001

First designer cellulosome

Engineered chimeric scaffoldin

Clostridium cellulolyticum, CelA, CelF

Cohesin-dockerin interaction

[3]

2003

Protein A- CBM -enzyme conjugates

Staphylococcal protein A

EGFP, Fusarium solani pisi lipase cutinase

IgG-affibody interaction

[4]

2009

Rosettazyme

Group II Chaperonin (Rosettazomes)

Clostridium thermocellum, Cel9B, Cel9K, Cel9R, Cel48S

Cohesin-dockerin interaction

[11]

2011

Nanocluster

Streptavidin-immobilized inorganic nanoparticles

Aspergillus niger, EglA

Avidin-biotin interaction

[12]

2012

Nanocluster

Polystyrene nanosphere

Trichoderma viride cellulase

EDC and Sulfo-NHS coupling chemistry

[13]

2013

Nanocluster

CdSe-ZnS core-shell quantum dots (QDs)

Clostridium thermocellum CelA, Clostridium cellulolyticum CelE

Metal affinity between core-shell QDs and polyhistidine tag

[14]

2013

Cellulase-ankyrin fusions

Ankyrin

Clostridium thermocellum CelA, Thermotoga marítima,, Cel12A

Protein fusion

[15]

2013

DNA-cellulase covalent conjugates

Double strand DNA

Thermobifida fusca, Cel5A

MTG mediated cross-linking

[16]

2014

DNA-cellulase non-covalent conjugates

Double strand DNA

Clostridium thermocellum, CelA

Zinc-finger protein guided assembly

[17]