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Sustainable Chemical Processes

Table 2 Binding (%) of ErL-ASNase to the affinity adsorbents

From: Extracellular expression and affinity purification of L-asparaginase from E. chrysanthemi in E. coli

Adsorbent

Binding (%)

L-Asn-Sepharose CL 6B

49

L-Asp-Sepharose CL 6B

71

L-Glu-Sepharose CL 6B

59

  1. Binding (%) was expressed as the proportion (%) of ErL-ASNase (U) bound on the affinity adsorbent to the overall units loaded. The standard deviation for the enzyme activities (U), evaluated from three independent determinations was found to be less than 10%.
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