Table 1 E. coli strains used in the present study
From: Extracellular expression and affinity purification of L-asparaginase from E. chrysanthemi in E. coli
E. colistrains | Genotypes | Supplier |
|---|---|---|
XL1-Blue | endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(rK− mK+) | Stratagene |
TOP10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ− | Invitrogen |
UT 5600 | F− ara-14 leuB6 secA6 lacY1 proC14 tsx-67 Δ(ompT-fepC)266 entA403 trpE38 rfbD1 rpsL109 xyl-5 mtl-1 thi-1 | New England Biolabs |
BL21(DE3) | F− ompT gal dcm lon hsdSB(rB− mB−) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Novagen |
BL21(DE3)Star | F− ompT gal dcm lon hsdSB(rB− mB−) λ(DE3),(cmR) | Invitrogen |
Rosetta(DE3) | F− ompT hsdSB(rB− mB−) gal dcm (DE3) pRARE (CamR) | Novagen |
BL21(DE3)pLysS | F− ompT gal dcm lon hsdSB(rB− mB−) λ(DE3) pLysS(cmR) | Novagen |