Two-step process for preparation of oligosaccharide propionates and acrylates using lipase and Cyclodextrin Glycosyl Transferase (CGTase)

Sustainable Chemical Processes

Table 1 Screening of organic solvent and immobilized lipases for transesterification of ethyl acrylate with glucose (G1) or maltose (G2)

		Dioxane (-1.1)	Acetonitrile (-0.36)	Acetone(-0.3)	Tetrahydrofurane(0.49)	Tert-butylalcohol(0.83)	Tert-amylalcohol(1.4)
C. antarctica (N435)	G1	++++	+++	++++	++++	+++	+++
C. antarctica (N435)	G2	++++	+	++++	+++	+	++
C. antarctica (MP1000)	G1	++++	++	++	++++	+++	+++
C. antarctica (MP1000)	G2	++++	++	++	-	++	++
T. lanuginosa (Lipozyme TL IM)	G1	++	++	+	++	++	+++
T. lanuginosa (Lipozyme TL IM)	G2	++	-	+	+++	+	++
T. lanuginosa (MP1000)	G1	-	-	-	-	-	-
T. lanuginosa (MP1000)	G2	-	-	-	-	-	-
M. miehei (MP1000)	G1	+	-	-	-	+	-
M. miehei (MP1000)	G2	-	-	-	-	-	-
C. rugosa (MP1000)	G1	-	-	-	-	-	-
C. rugosa (MP1000)	G2	-	-	-	-	-	-

Product formation is expressed using the following scale to describe the color intensity of the product spots on TLC: (-) not detected; (+) weak; (++) intermediate; (+++) strong; (++++) very strong.