Table 2 Box-Benhken Design matrix with experimental and predicted L-asparaginase activity

1

4

30

35

7

4.710

4.688

2

8

30

35

7

4.655

4.860

3

4

50

35

7

2.912

3.729

4

8

50

35

7

4.790

5.829

5

6

40

30

6

3.458

3.802

6

6

40

40

6

3.493

3.647

7

6

40

30

8

3.352

4.220

8

6

40

40

8

3.534

4.212

9

4

40

35

6

3.827

3.279

10

8

40

35

6

3.504

3.512

11

4

40

35

8

3.305

2.866

12

8

40

35

8

4.790

4.907

13

6

30

30

7

5.354

4.922

14

6

50

30

7

6.182

5.370

15

6

30

40

7

4.902

5.284

16

6

50

40

7

4.843

4.846

17

4

40

30

7

3.264

3.813

18

8

40

30

7

4.338

4.058

19

4

40

40

7

2.823

2.840

20

8

40

40

7

5.683

4.869

21

6

30

35

6

4.174

4.579

22

6

50

35

6

4.679

4.634

23

6

30

35

8

5.342

5.120

24

6

50

35

8

5.747

5.075

25

6

40

35

7

5.858

5.169

26

6

40

35

7

5.013

5.169

27

6

40

35

7

4.802

5.169

28

6

40

35

7

4.855

5.169

29

6

40

35

7

5.049

5.169