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Table 3 Insulin aggregation assay for thioredoxin proteins

From: Microwave assisted solubilization of inclusion bodies

  Protein (mg) Activity (Units)* Specific-Activity (Units mg-1)
Initial protein taken for refolding (solubilized inclusion bodies) 0.50 - -
Affinity precipitation refolded malETrx 0.32 1.10 3.4
WT-Thioredoxin (Sigma-Aldrich) - - 4.0
  1. The experiments were carried out in triplicates and the difference in the individual readings was less than 5%.
  2. *One unit will cause a ΔA650 of 1.0 in 1 min at 25°C.