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Table 2 Effect of microwave treatment on the average sizes of different inclusion bodies dissolved in different denaturants

From: Microwave assisted solubilization of inclusion bodies

Inclusion Bodies Average diameter (nm) of inclusion bodies in buffer before microwave treatment Average diameter (nm) after microwave treatment.
   8 M urea 6 M GuHCl 20 mM SDS
MBP224D 1493 390 451 130
MBP264D 1504 440 326 130
CcdB-M97K 751 93 438 62
CD4D12 1040 284 338 295
MalETrx 731 120 410 195
ScFv 1444 615 587 560
ScFab 1400 884 811 547
  1. Inclusion bodies of MBP224D, MBP264D, CcdB-M97K, CD4D12, malETrx, ScFv and ScFab, were dissolved in 8 M urea, 6 M GuHCl and 20 mM SDS and subjected to microwave treatment for 2 minutes and the average diameter was recorded. The average diameters of inclusion bodies suspended in 50 mM Tris–HCl, pH 7.0 (without denaturant) and prior to microwave treatment were also recorded for comparison. The average sizes of the dissolved inclusion bodies were measured by dynamic light scattering. In all the measurements the buffer used was 50 mM Tris–HCl, pH 7.0. The temperature during microwave solubilization was maintained at 26 ± 1°C. Protein concentration in all the cases was 2.0 mg.mL-1. The readings were taken in triplicates and the difference among the individual readings was less than 5%.