|
Denaturant
|
Average diameter (nm) before microwave treatment
|
Average diameter (nm) after microwave treatment
|
|---|
|
8 M urea
|
706
|
587
|
|
6 M GuHCl
|
110
|
9
|
|
20 mM SDS
|
422
|
7
|
- The denaturants 8 M urea, 6 M GuHCl and 20 mM SDS were prepared in 50 mM Tris–HCl, pH 7.0 and their average sizes (nm) were recorded before and after microwave treatment (for 2 minutes), by dynamic light scattering. This was done as a control experiment. The temperature during microwave solubilization was maintained at 26 ± 1°C. The readings were taken in triplicates and the difference among the individual readings was less than 5%.
