Microwave assisted solubilization of inclusion bodies

Sustainable Chemical Processes

Table 1 Average sizes of different denaturants

Denaturant	Average diameter (nm) before microwave treatment	Average diameter (nm) after microwave treatment
8 M urea	706	587
6 M GuHCl	110	9
20 mM SDS	422	7

The denaturants 8 M urea, 6 M GuHCl and 20 mM SDS were prepared in 50 mM Tris–HCl, pH 7.0 and their average sizes (nm) were recorded before and after microwave treatment (for 2 minutes), by dynamic light scattering. This was done as a control experiment. The temperature during microwave solubilization was maintained at 26 ± 1°C. The readings were taken in triplicates and the difference among the individual readings was less than 5%.