Figure 5

Fluorescence based maltose binding assay for refolded MBP264D. The inclusion bodies were solubilized by microwave assistance in 2 min with 8 M urea before refolding by using cationic starch as a smart polymer. Fluorescence emission spectra (AU, arbitrary units) were acquired in 10 mM Tris–HCl, pH 7.5; using excitation and emission slit widths of 2 nm and 5 nm, respectively. Protein samples at a concentration of 3.5 μg/mL^-1 were incubated in Tris buffer (—) and in Tris buffer containing 0.1 mM maltose (− − −) 15 min before spectral acquisition.