Figure 4

Fluorescence emission spectra of refolded proteins. The inclusion bodies were solubilized by microwave assistance in 2 min with 8 M urea before the refolding process. All the spectra were recorded with 1 μM protein concentration in 10 mM Tris–HCl, pH 7.5, with excitation at 280 using excitation and emission slit widths of 2 nm and 5 nm, respectively: (A) Refolded malETrx (thioredoxin) and (B) refolded CcdB-M97K. In both the figures, the two curves show: refolded protein (—) and unfolded protein in 8 M urea (− − −).