Sizes of inclusion bodies of MBP264D with and without microwave assistance. The inclusion bodies were dissolved in different denaturants in 24 hours at 25°C. The same dissolution was achieved with microwave treatment in 2 minutes. The average sizes of the dissolved inclusion bodies were measured by dynamic light scattering. Solutions of all the denaturants were prepared in 50 mM Tris–HCl, pH 7.0.