Figure 2

Effect of water content. Crude coffee oil (0.5 g) and ethanol were taken in the ratio of 1:4 (mol/mol). 10% (w/w of oil) Novozym 435 was added. Different amounts of water (w/w of enzyme) were added to the reaction media. Aliquots were taken out after 4 h and analyzed by GC as described in materials and methods section. The experiments were done in duplicates and the error between the two sets was within 3%.