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Sustainable Chemical Processes

Table 3 Insulin aggregation assay for thioredoxin proteins

From: Microwave assisted solubilization of inclusion bodies

 

Protein (mg)

Activity (Units)*

Specific-Activity (Units mg-1)

Initial protein taken for refolding (solubilized inclusion bodies)

0.50

-

-

Affinity precipitation refolded malETrx

0.32

1.10

3.4

WT-Thioredoxin (Sigma-Aldrich)

-

-

4.0

  1. The experiments were carried out in triplicates and the difference in the individual readings was less than 5%.
  2. *One unit will cause a ΔA650 of 1.0 in 1 min at 25°C.
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